Nanobacteria-like particles in human arthritic synovial fluids.

نویسندگان

  • T Tsurumoto
  • T Matsumoto
  • A Yonekura
  • H Shindo
چکیده

We investigated the existence of nanosize particles in synovial fluids of rheumatoid arthritis and osteoarthritis patients. These specimens were cultured under mammalian cell culture conditions (37 degrees C; 5% CO2/95% air) for a long period. After about 2 months, many nanoparticles appeared and they gradually increased in number and in size. The nanobacteria-like particles exist in synovial fluids of arthritis patients. The possibility of their existence and pathogenesis in various diseases should be verified cautiously.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Synovial fluid inflammatory cytokines and proteins in clinically healthy and arthritic joint of dromedary camels (Camelus dromedarius)

Analyzing the synovial fluid is one of the common methods to diagnose the articular problems to detect the stage and express the prognosis. Such gross, cytological, and biochemical analysis of synovial fluids can aid in the diagnosis of various joint diseases. Objectives: Normal values for synovial fluid in the camels have been described previously; however, there are no reports regarding conce...

متن کامل

The detection, quantification and partial characterisation of cathepsin B-like activity in human pathological synovial fluids.

In this study, the levels of the cysteine proteinase--cathepsin B were measured in diseased synovial fluids using a steady state fluorometric assay. Cathepsin B-like activity was shown to be present in all the samples analysed, with the rheumatoid arthritic synovial fluids possessing significantly higher concentrations (mean value ca. 416 mg/l) than the osteoarthritic fluids (mean value ca. 142...

متن کامل

Critical Evaluation of Gamma-Irradiated Serum Used as Feeder in the Culture and Demonstration of Putative Nanobacteria and Calcifying Nanoparticles

The culture and demonstration of putative nanobacteria (NB) and calcifying nanoparticles (CNP) from human and animal tissues has relied primarily on the use of a culture supplement consisting of FBS that had been gamma-irradiated at a dose of 30 kGy (gamma-FBS). The use of gamma-FBS is based on the assumption that this sterilized fluid has been rid entirely of any residual NB/CNP, while it cont...

متن کامل

Identification of human inflammatory dendritic cells

Dendritic cells (DCs) are professional antigen-presenting cells that comprise several subsets with distinct phenotypes and functions, including inflammatory DCs that appear during inflammation. By analyzing human inflammatory fluids (arthritic synovial fluid and tumor ascites), we have identified the human equivalent of inflammatory DCs.

متن کامل

Spectrophotometric measurement of proteoglycans in osteoarthritic synovial fluid.

Cartilage glycosaminoglycans (GAGs) were measured by a spectrophotometric assay in synovial fluid obtained from 30 normal bovine hock joints and 15 osteoarthritic human knee joints. Results were compared with those obtained by radioimmunoassay (RIA). The spectrophotometric method (dimethylmethylene blue (DMB) assay) was found to be simple, safe, and sufficiently reproducible to be of potential ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of proteome research

دوره 5 5  شماره 

صفحات  -

تاریخ انتشار 2006